Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Arid2

Cell type

Cell type Class
Others
Cell type
OP9
Tissue
Bone Marrow/Stroma
Cell Type
Embryonic Stem Cells, Macrophage

Attributes by original data submitter

Sample

source_name
shCtrl
cell line
OP9
cell type
Stromal Cell line
chip antibody
ARID2 (Bethyl Laboratories, Cat. A302-230A , Lot A302-230A-1)
knockdown
shCtrl

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were precipitated with specific antibody. ChIP-seq library preparation was performed using TruSeqChIP Sample Prep Kit (Illumina) according to the manufacturer's instructions.10ng of input ChIP-enriched DNA was used for ChIP-seq library preparation. Final libraries were checked using High Sensitivity chips in 2100 Bioanalyzer (Agilent). Average fragment size of final libraries was found to be 277 ± 13bp. Paired-end sequencing (2 x 100 bp) of these libraries were performed in HiSeq-2500 (Illumina) according to the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
47956225
Reads aligned (%)
95.1
Duplicates removed (%)
75.7
Number of peaks
312 (qval < 1E-05)

mm9

Number of total reads
47956225
Reads aligned (%)
95.0
Duplicates removed (%)
75.9
Number of peaks
283 (qval < 1E-05)

Base call quality data from DBCLS SRA